Many varieties of geranium carry viruses that do not show symptoms(cheap seed starting trays wholesale greece). The plants produced by the shoot tip culture are more vigorous than the untreated plants, and the number of cuttings produced is 20% to 30%, and these cuttings are easy to root(288 cell plug tray). The benefits of this technology have been valued by relevant government departments and producers.
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The more complex the nutrients needed for the young embryo gold, except for the General inorganic salts(51 cell seed trays). Generally speaking, the specific operation methods are as follows: wash the young fruits or seeds with tap water, put them into saturated bleaching powder supernatant for disinfection for 10 ~ 30 minutes, or put them into 0.1% mercuric solution for disinfection for 10 ~ L5 minutes(cheap seed starting trays wholesale greece).
The seed coat of some flowers is hard, so it is difficult to remove the seed coat and peel off the embryo(12.5 cm plant pots). Soak it in 0.in sodium hydroxide solution for 1 ~ 5 minutes before alcohol poisoning, then rinse it with clean water for 20 ~ 30 minutes, and then disinfect it(13cm plastic plant pots). The selection of appropriate medium is the most important condition to ensure the continuous embryonic growth and germination of embryos(cheap seed starting trays wholesale greece).
If such embryos are taken out at an appropriate time and cultured on an artificial medium, it is possible for the immature embryos to develop and grow normally to obtain offspring of the stripe, thereby shortening the breeding cycle in production(50 cell propagation trays). There are many nucellar embryos in citrus plants, and the viability of nucellar embryos is very strong, while the viability of hybrid embryos is low(cheap seed starting trays wholesale greece).
Sterile test tube plantlets formed by embryo culture such as Clivia and Orchid(105 cell plug flats). Generally, if zygotic embryos are taken out and cultured on artificial culture medium at an early stage, it is possible to obtain more than varieties of offspring, so as to select excellent poor varieties(cheap seed starting trays wholesale greece). In the past 20 years, with the rapid development of plant tissue culture technology, embryo culture has been widely used(105 cell plug trays).
Or soak the seeds in hot water at 45 ~ 90 ℃ and let them cool naturally to make the seeds fully absorb water, then disinfect, peel off the seed coat and take out the embryo for use(cheap seed starting trays wholesale greece). Amino acids, vitamins and natural extracts should also be added to meet the needs of normal embryo growth(200 cell seed trays), while large and nearly mature embryos can grow normally as long as inorganic salts and sucrose are added.
In this way(12cm nursery pot), by introducing known viruses into virus-free plants to compare the performance of susceptible plants and virus-free plants of the same clone, it significantly shows that the virus causes a substantial decline in the viability, quality and yield of the host plant(cheap seed starting trays wholesale greece). After pollination, the sperm and egg combine to produce a conjugator, and the conjugator undergoes multiple divisions to produce an embryo.
At this time, the stem tip is transferred to the basic medium without growth regulators to continue the cultivation, the stem tip is continuously elongated(seedling tray 104 holes), the leaves are expanded, and the root system is formed at the base of the stem, and finally develops into a complete plantlet(cheap seed starting trays wholesale greece). The culture conditions play an important role in whether embryos can grow normally in the culture medium(128 cell seed trays).
At present, the ultra-low temperature preservation technology of plant cells, tissues and organs has developed very fast(162 cell seed starting trays). Then the size of the stem center in vitro is a key factor. On the one hand, stem inactivation culture technology can increase the yield of crops, cash crops and poor plants and greatly improve their quality(cheap seed starting trays wholesale greece); On the other hand, it can promote the import and export of living plant materials.
The land is applied to the production and breeding of flowers and forests, and has great application value(32 cell seed tray). Due to the requirements of ultra-low temperature preservation, such conditions as dense protoplasm, no vacuolization and thin cell wall are required, and the young embryos have these favorable conditions, so they have achieved good results(cheap seed starting trays wholesale greece). After disinfection, it must be washed with sterile water for 3 ~ 4 times.
all know that the embryonic development of dicot plants generally goes through five stages(98 cell seed tray): protoglobular embryo, heart-shaped embryo, torpedo-shaped embryo, cotyledon-shaped embryo and mature embryo. Mature embryo has four parts: cotyledon, embryo, radicle and hypocotyl(cheap seed starting trays wholesale greece). Embryo culture can overcome seed dormancy and shorten the seedling time(72 cell plug trays). Some flower seeds have a long dormancy period.
Embryo culture is to use artificial methods to separate the embryo from the seed, place it on the culture medium for cultivation, make it develop into a complete seedling(50 cell plug trays), and then transplant it into the soil to make it grow normally, bloom and bear fruit(cheap seed starting trays wholesale greece). It is reported that a large number of sterile seedlings can be obtained through the test tube culture of embryos, providing sterile explant materials for rapid propagation.
For example, the dormancy period of iris seeds is as long as 2 to 3 years(21 cell seed trays). If the embryo is taken out and placed on an artificial medium, it will be cultured within a few days(cheap seed starting trays wholesale greece). It can germinate and develop smoothly into seedlings with roots and leaves. Using this technology can greatly shorten the growth cycle from seed to flowering(32 cell seedling tray). Therefore, when choosing the light source of the dissecting mirror, a blind light is better.
Using embryo culture can solve the problem of hybrid sterility in distant hybridization(cheap seed starting trays wholesale greece). It is known that in distant hybridization, the embryo is often aborted due to incomplete embryo development, or the embryo cannot germinate, so that the distant hybridization between genera and species cannot be successful(128 cell plug trays). The offspring of Citrus dead species with high ornamental value are obtained through embryo culture.
We usually use the embryo culture of seeds or fruits that takes half the time from pollination to maturity, which is easy to succeed(cheap seed starting trays wholesale greece). More and more countries stipulate that only virus-free plants certified by inspection can be imported(15 cell seed trays). Therefore, nucellar embryos often inhibit the development of zygotic embryos and make them unable to grow normally, which brings great difficulties to citrus dead hybrid breeding.
Some saprophytic or parasitic plants(72 cell propagation tray), such as orchids and Gastrodia elata, when the host does not exist, in order to prevent some rare and precious varieties from becoming extinct, the embryos can be isolated from their seeds and cultured on a medium supplemented with appropriate hormones and organic growth substances(9cm flower pots), so that the embryos may germinate in large numbers and continue to produce offspring(cheap seed starting trays wholesale greece).
