Then place the mouth of the tube (bottle) and the tampon on the lamp flame and rotate for a few seconds (be careful not to burn the tampon), then close the tampon tightly, wrap it with wrapping paper, and tie the tube tightly with a cotton rope or rubber band (Bottle) mouth(5 inch plastic plant pots suppliers malaysia). The role of light is mainly in the induction of morphogenesis, and it involves some important processes of plant cell differentiation.
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Therefore, when doing the single factor test of pH value, the magnitude difference should not be too small, otherwise the test result is not obvious(plastic pots that look like terracotta). In general, as long as the growth and proliferation of the culture can meet the requirements of rapid propagation, it should be noted that all hormone types and their ratio tests should be repeated for 2 ~ 3 times on the same medium to determine the effect(5 inch plastic plant pots suppliers malaysia).
If MS medium is used, cytokinin BA (0.5 ~ 5mgl) and auxin NAA (0.1 ~ 1.0mgl) can be added(5 inch plastic plant pots suppliers malaysia). The culture temperature is 24 ~ 26 ℃, and the light is illuminated for 10 hours every day(4 inch square nursery pots). Generally speaking, this medium and culture conditions can make hundreds of plants that are easy to regenerate differentiate and proliferate smoothly(v10 nursery pots). A single factor test with variable pH value can also be used to find the appropriate pH.
After a period of culture, revise and adjust the amount of plant hormone in the next culture medium according to the performance of most cultures(thermoform pots). For newly cultivated flower species, refer to the H value of similar plants published in the past(v13 nursery pots). Some materials themselves have more thread, oil, wax and thorns(5 inch plastic plant pots suppliers malaysia). After washing with tap water for a long time, the rate of bud formation is low below 12°C and above 33°C(4x8 grow tray).
Two groups of single factor experiments can also be used to select different hormone dosage(5 inch plastic plant pots suppliers malaysia): the first group finds out the appropriate dosage of cytokinin B (MGL), and the second group finds out the dosage of auxin NAA (MGL). In this group of single factor experiments, the dosage of NAA remains unchanged, and pay attention to the effect of Ba on culture(plastic plant pots ireland). Usually the sugar concentration is selected in a small range.
In anther culture, the range of change is large, sometimes 70~100gL is used(plastic flower pots canada). When retesting, the hormone ratio of MS + Ba2 + naa0.5 can be selected. Knives and tweezers should be cleaned with alcohol cotton every moment of use, and they should be burned and kept cool for standby(5 inch plastic plant pots suppliers malaysia). Knives and tweezers are often exchanged for use, which can not only improve work efficiency, but also prevent continuous pollution(plug flats).
The mixture of formaldehyde and potassium permanganate should be used for air vertical steam sterilization regularly(5 inch plastic plant pots suppliers malaysia). In addition, in the process of culture, the pH value of the medium will change with the consumption of nutrients(8 inch nursery pots). These articles also have ideal use effects. Experiments have proved that this process is to collect the "feedback information" of the culture on the culture medium and culture conditions.
Therefore, especially for some perennial woody plant materials, in the preparatory test, the change range of hormone dosage can be larger(v11 nursery pots). After selecting a better combination, reduce the magnitude. After several rounds of tests, find the most suitable hormone ratio step by step(5 inch plastic plant pots suppliers malaysia). In the hormone type and ratio test, KT, ZT, 2-ip, etc(v15 nursery pots). can also be selected for cytokinin, and IAA, IBA, 2.4-D, etc. can also be selected for auxin(72 cell propagation tray).
It should be noted that the pH value of the medium will generally decrease by 0.5 ~ 1.0 units during sterilization(5 inch plastic plant pots suppliers malaysia). The culture temperature of proliferating seedlings is generally at a constant temperature, and there are some cases where the growth effect under the changing temperature of day and night is better than that of constant temperature, such as lilies(plug trays canada). The stems and leaves of plants are mostly exposed to the air.
The main source of pollution in inoculation room and culture room is bacteria and fungal spores in the air(plastic planters canada). There is no need to pursue a precise pH value too much(5 inch plastic plant pots suppliers malaysia). Then use the thumb, middle finger and index finger of your right hand to hold a piece of explant into the tube (problem) with forceps, gently insert it into the culture medium, burn the forceps and put it back on the stent, and then gently plug the tampon.
They are available on the market without self-made, easy to use, save working hours and high efficiency(5 inch plastic plant pots suppliers malaysia). Plants do not have very strict requirements on pH, just use pH 5.6~5.8(20 inch plastic planter). Some acid-loving flower plants, such as camellia, rhododendrons, bougainvillea and African violets, can appropriately lower the pH value from 5.4 to 5.0(6 inch plastic plant pots). For most plants, the appropriate sugar concentration is 20~30gL, and 40gL is used in individual cases.
The temperature to promote bud formation is generally slightly lower, preferably 15~18°C(2.5 inch succulent pot). When considering the temperature requirements of a certain culture, the temperature conditions of the original plant's ecological environment should be considered. If some plants grow at a higher altitude and a lower environmental temperature, their test tube should be cultured at a higher temperature(5 inch plastic plant pots suppliers malaysia). Seedlings grow slowly.
In the early stage of shoot tip culture, high temperature treatment not only can obtain virus-free plants, but also affects organogenesis(v9 nursery pots). The worst(5 inch plastic plant pots suppliers malaysia). Bulbous sea purple test-tube plantlets grow poorly in a culture environment above 30°C(3x6 grow tray). For example, the stem apex meristem of strawberry is treated with a high temperature of 38 ℃ for 3 to 5 days, which improves the survival rate of the virus-free stem apex meristem.
The suitable temperature for tissue culture of most of the blooming plants is 22~28℃(5 inch plastic plant pots suppliers malaysia). The temperature of 25℃±2℃ is adopted(plastic plant pots price). If it is lower than 15°C, the growth of explants will be stagnant, and higher than 35°C is also detrimental to growth(7 inch plastic flower pots). If the culture temperature is lowered to simulate the low temperature environment in winter for 2 to 3 months, the growth rate of the test tube seedlings will return to normal.
And a variety of Pelargonium was treated with different temperatures such as 10℃, 20℃ and 30℃ for 1~4 weeks(4 inch plastic plant pots). After 8 weeks of cultivation, it was found that the number of shoot tip reproduction was highest when treated at 10℃, followed by treatment at 20℃, followed by treatment at 30℃(5 inch plastic plant pots suppliers malaysia). They are contaminated by soil, fertilizer and other bacteria during cultivation, so they must be sterilized before being sterilized.
